Back to Search
Start Over
Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection
- Source :
- Veterinary Research, Vol 50, Iss 1, Pp 1-14 (2019)
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Abstract Enterotoxigenic Escherichia coli (ETEC) are an important cause of post-weaning diarrhea (PWD) in piglets. The IL-17 cytokine family is well known to play important roles in the host defense against bacterial infections at the mucosa. Previously, we reported the potential role of IL-17A in clearing an ETEC infection in piglets. IL-17C, another member of the IL-17 family, is highly expressed in the intestinal epithelium, however, its role during an ETEC infection is still unclear. In this study, we demonstrate that F4+ ETEC induce IL-17C mRNA and protein expression in intestinal tissues as well as in porcine intestinal epithelial cells (IPEC-J2). This IL-17C production is largely dependent on TLR5 signaling in IPEC-J2 cells. Both F4+ ETEC infection and exogenous IL-17C increased the expression of antimicrobial peptides and tight junction proteins, such as porcine beta-defensin (pBD)-2, claudin-1, claudin-2 and occludin in IPEC-J2 cells. Taken together, our data demonstrate that TLR5-mediated IL-17C expression in intestinal epithelial cells enhances mucosal host defense responses in a unique autocrine/paracrine manner in the intestinal epithelium against ETEC infection.
- Subjects :
- Veterinary medicine
SF600-1100
Subjects
Details
- Language :
- English
- ISSN :
- 12979716
- Volume :
- 50
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Veterinary Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.1c82d3cd9a754f408140fda40fd564e4
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s13567-019-0665-8