Back to Search Start Over

GraS is critical for chloroplast development and affects yield in rice

Authors :
Zhi-xuan DU
Hui-ying HAO
Jin-peng HE
Jian-ping WANG
Zhou HUANG
Jie XU
Hai-hui FU
Jun-ru FU
Hao-hua HE
Source :
Journal of Integrative Agriculture, Vol 19, Iss 11, Pp 2603-2615 (2020)
Publication Year :
2020
Publisher :
Elsevier, 2020.

Abstract

Leaf color has been considered an important agronomic trait in rice (Oryza sativa L.) for a long time. The changes in leaf color affect the yield of rice. In this study, a green-revertible albino (graS) mutant was isolated from a 60Co-gamma-irradiated mutant pool of indica cultivar Guangzhan 63-4S. The fine mapping indicated that graS mutant was mapped to chromosome 1, and was located in a confined region between markers ab134 and InDel 8 with genetic distances of 0.11 and 0.06 cM, respectively. Based on the annotation results, four open reading frames (ORFs) were predicted in this region. Sequence analysis revealed that LOC_Os01g55974 had a 2-bp nucleotide insertion (AA) in the coding region that led to premature termination at the 324th base. Sequence analysis and expression analysis of related genes indicated that LOC_Os01g55974 is the candidate gene of GraS. We studied the genome and protein sequences of LOC_Os01g55974, and the data showed that GraS contains a deoxycytidine deaminase domain, which was expressed ubiquitously in all tissues. Further investigation indicated that GraS plays an essential role in the regulation of chloroplast biosynthesis, photosynthetic capacity and yield. Moreover, leaf color mutant can be used as an effective marker for the purity of breeding and hybridization.

Details

Language :
English
ISSN :
20953119
Volume :
19
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Journal of Integrative Agriculture
Publication Type :
Academic Journal
Accession number :
edsdoj.1b756c7dec349a8bb2ae4ae2b9694dc
Document Type :
article
Full Text :
https://doi.org/10.1016/S2095-3119(19)62859-5