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Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus

Authors :
Ma. Del Rocio Baños-Lara
Jovanny Zabaleta
Jone Garai
Melody Baddoo
Antonieta Guerrero-Plata
Source :
BMC Research Notes, Vol 11, Iss 1, Pp 1-7 (2018)
Publication Year :
2018
Publisher :
BMC, 2018.

Abstract

Abstract Objective Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are responsible for respiratory diseases, mostly in children. Despite the clinical and epidemiological similarities between these two pneumoviruses, they elicit different immune responses. This work aims to further our understanding of the differential immune response induced by these respiratory viruses by determining the changes of small non-coding RNAs (miRNAs), which regulate gene expression and are involved in numerous cellular processes including the immune system. Results In the present study, we analyzed the expression of miRNA transcripts of human dendritic cells infected with RSV or HMPV by high throughput sequencing using Illumina sequencing technology. Further validation of miRNA expression by quantitative polymerase chain reaction indicated that HMPV infection up-regulated the expression of 2 miRNAs (hsa-miR-182-5p and hsa-miR-4634), while RSV infection induced significant expression of 3 miRNAs (hsa-miR-4448, hsa-miR-30a-5p and hsa-miR-4634). The predominant miRNA induced by both viruses was hsa-miR-4634.

Details

Language :
English
ISSN :
17560500
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Research Notes
Publication Type :
Academic Journal
Accession number :
edsdoj.1b6cb9b86c6d4959aac3a5116b6f68f8
Document Type :
article
Full Text :
https://doi.org/10.1186/s13104-018-3541-0