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Synthesis, Characterization, and Biological Evaluation of Nanostructured Hydroxyapatite with Different Dimensions

Synthesis, Characterization, and Biological Evaluation of Nanostructured Hydroxyapatite with Different Dimensions

Authors :
Zhen Geng
Qin Yuan
Xianglong Zhuo
Zhaoyang Li
Zhenduo Cui
Shengli Zhu
Yanqin Liang
Yunde Liu
Huijing Bao
Xue Li
Qianyu Huo
Xianjin Yang
Source :
Nanomaterials, Vol 7, Iss 2, p 38 (2017)
Publication Year :
2017
Publisher :
MDPI AG, 2017.

Abstract

Nanosized hydroxyapatite (HA) is a promising candidate for a substitute for apatite in bone in biomedical applications. Furthermore, due to its excellent bone bioactivity, nanosized strontium-substituted HA (SrHA) has aroused intensive interest. However, the size effects of these nanoparticles on cellular bioactivity should be considered. In this study, nanosized HA and SrHA with different dimensions and crystallization were synthesized by hydrothermal methods. The phase, crystallization and chemical composition were analyzed by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR), respectively. The morphology was observed under field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The degradation behaviors of the samples were monitored by determining the ions release profile with inductively coupled plasma mass spectrometry (ICP-MS). The releasing behavior of Ca2+ and Sr2+ showed that the degradation rate was proportional to the specific surface area and inversely proportional to crystallization. The in vitro experiment evaluated by MG63 cells showed that SrHA nanorods with a length greater than 100 nm had the best biological performance both in cell proliferation and differentiation (* p < 0.05 compared with HA-1 and SrHA-1; * p < 0.01 compared with HA-2). In addition, HA nanoparticles with a lower aspect ratio had better bioactivity than higher ones (* p < 0.05). This study demonstrated that nanosized HA and SrHA with subtle differences (including dimensions, crystallization, specific surface area, and degradation rate) could affect the cellular growth and thus might have an impact on bone growth in vivo. This work provides a view of the role of nano-HAs as ideal biocompatible materials in future clinical applications.

Details

Language :
English
ISSN :
20794991
Volume :
7
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Nanomaterials
Publication Type :
Academic Journal
Accession number :
edsdoj.19eb67e367ba4d618e0d7b029524be00
Document Type :
article
Full Text :
https://doi.org/10.3390/nano7020038