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Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS
- Source :
- BioTechniques, Vol 48, Iss 1, Pp 61-64 (2010)
- Publication Year :
- 2010
- Publisher :
- Future Science Ltd, 2010.
-
Abstract
- We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10% sarkosyl effectively solubilized >95% of proteins, while high-yield recovery of sarkosyl-solubilized fusion proteins was obtained with a specific ratio of Triton X-100 and CHAPS. We demonstrate for the first time that this combination of three detergents significantly improves binding efficiency of GST and GST fusion proteins to gluthathione (GSH) Sepharose.
Details
- Language :
- English
- ISSN :
- 19409818 and 07366205
- Volume :
- 48
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- BioTechniques
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.195fae27bbf8457fbd0a551939110b8b
- Document Type :
- article
- Full Text :
- https://doi.org/10.2144/000113304