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Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.
- Source :
- PLoS ONE, Vol 10, Iss 10, p e0139695 (2015)
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- We developed and optimized a high-throughput project workflow to generate renewable recombinant antibodies to human proteins involved in epigenetic signalling. Three different strategies to produce phage display compatible protein antigens in bacterial systems were compared, and we found that in vivo biotinylation through the use of an Avi tag was the most productive method. Phage display selections were performed on 265 in vivo biotinylated antigen domains. High-affinity Fabs (
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 10
- Database :
- Directory of Open Access Journals
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.18dddf47c540eb9b460223ee62eb80
- Document Type :
- article
- Full Text :
- https://doi.org/10.1371/journal.pone.0139695