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Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD
- Source :
- Journal of Spectroscopy, Vol 2017 (2017)
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Nelumbo nucifera has a variety of biological activities. So it was importantly used as various herbal medicines since traditional times. A simple, fast, and sensitive high-performance liquid chromatographic (HPLC) method was developed in this study for efficient quality control of N. nucifera. Four different compounds, including neferine, 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol, 1-hydroxy-2-methylpropene, and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol, were simultaneously determined. The four compounds were isolated through a Dionex C18 column by gradient elution with 0.1% TFA-water and methanol. The flow rate was 1.0 mL/min, and the wavelength was detected at 205, 254, 280, and 330 nm. The chromatograms were acquired at 205 nm. The four compounds showed good linear relationships (r2>0.96) over five different concentrations, and an average recovery of the method ranged from 96.27% to 108.78%. Through the analysis validation test and application of the method, the optimized conditions verified that it is efficient to isolate the compounds of N. nucifera seed embryos.
- Subjects :
- Optics. Light
QC350-467
Subjects
Details
- Language :
- English
- ISSN :
- 23144920 and 23144939
- Volume :
- 2017
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.17a07956d75849cfb1efd70c6bb3c97b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1155/2017/6426394