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Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
- Source :
- Food and Agricultural Immunology, Vol 29, Iss 1, Pp 147-158 (2018)
- Publication Year :
- 2018
- Publisher :
- Taylor & Francis Group, 2018.
-
Abstract
- In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.
Details
- Language :
- English
- ISSN :
- 09540105 and 14653443
- Volume :
- 29
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Food and Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.177eb9bd7eab4d3f8afbb544b70847c0
- Document Type :
- article
- Full Text :
- https://doi.org/10.1080/09540105.2017.1364706