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Water filter: a rapid water environmental DNA collector in the field

Authors :
Ping Wu
Jie Feng
Mingxia Ju
Shenhao Wu
Weichun Han
Miao Wang
Junquan Liao
Lifeng Zhao
Yifan Gao
Jiao Zheng
Mingjie Luo
Huixian Gong
Lidong Zeng
Juan Lai
Mingze Li
Qin Yan
Lei Sun
Yongfeng Liu
Source :
Frontiers in Environmental Science, Vol 12 (2024)
Publication Year :
2024
Publisher :
Frontiers Media S.A., 2024.

Abstract

Biological monitoring using environmental DNA (eDNA) technology has expanded from micro- to macro-organisms. In aquatic eDNA studies, large volumes of water need to be filtered rapidly in the field, which requires development of effective eDNA collection devices. In this study, we introduce a novel portable eDNA collection system containing a GM dual-channel water filter and a DNA extraction kit adapted to large filter membranes (ø 100 mm). The water filter is powered by a high-capacity lithium battery (9,000 mA), which operates two peristaltic pumps and maintains a continuous filtration rate of up to 1 L/min for 5 h in outdoor settings. For sample collection, the optimum conditions are still water and turbidity below 8 nephelometric turbidity units. This allows for the filtration of 10 L of water within 10 min by use of a 0.22-μm filter. Metagenomic and 12S metabarcoding sequencing showed that the DNA extraction quality and species annotation accuracy of our custom DNA extraction kit, which was tailored for this system, rivaled the performance of established kits. The GM water filter’s enrichment mode gave consistent results with vacuum filtration, which greatly reduced the filtration time for large water samples, while accurately reproducing species annotations. This innovation streamlines the eDNA collection and annotation process and offers substantial benefits for biodiversity monitoring and conservation efforts.

Details

Language :
English
ISSN :
2296665X
Volume :
12
Database :
Directory of Open Access Journals
Journal :
Frontiers in Environmental Science
Publication Type :
Academic Journal
Accession number :
edsdoj.172cc694d3e440b883f1578779455cef
Document Type :
article
Full Text :
https://doi.org/10.3389/fenvs.2024.1415338