Physicochemical and Anti-UVB-Induced Skin Inflammatory Properties of Lacticaseibacillus paracasei Subsp. paracasei SS-01 Strain Exopolysaccharide

The exopolysaccharide secreted by Lacticaseibacillus paracasei subsp. paracasei SS-01 strain (LP-EPS) is isolated and purified from yogurt. It is a polysaccharide with a branched and multi-stranded structure, which exists in a smooth rod-like or cloud-like state, and possesses a good thermal stability and a molecular weight of 49.68 kDa (±4.436%). LP-EPS shows a high antioxidant capacity, anti-inflammatory and anti-sensitizing activity during in vitro experimental studies, with half clearance (IC50) rates of 0.449, 1.314, and 2.369 mg/mL for the ABTS, DPPH, and OH radicals, respectively, and a half inhibition rate (IC50) of hyaluronidase of 1.53 mg/mL. A cell-based assay, enzyme-linked immunosorbent assay (ELISA), and quantitative real-time fluorescence PCR (qRT-PCR) show that LP-EPS effectively treats or ameliorates the skin inflammatory responses triggered by UVB irradiation, as evidenced by a highly significant decrease in the secretion of inflammatory factors by human skin keratinocytes (HaCaT), and a highly significant downregulation of the mRNA expression of MAPK/AP-1 pathway cytokines.