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Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing

Authors :
Amin Allahyar
Mark Pieterse
Joost Swennenhuis
G. Tjitske Los-de Vries
Mehmet Yilmaz
Roos Leguit
Ruud W. J. Meijers
Robert van der Geize
Joost Vermaat
Arjen Cleven
Tom van Wezel
Arjan Diepstra
Léon C. van Kempen
Nathalie J. Hijmering
Phylicia Stathi
Milan Sharma
Adrien S. J. Melquiond
Paula J. P. de Vree
Marjon J. A. M. Verstegen
Peter H. L. Krijger
Karima Hajo
Marieke Simonis
Agata Rakszewska
Max van Min
Daphne de Jong
Bauke Ylstra
Harma Feitsma
Erik Splinter
Wouter de Laat
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Preservation of cancer biopsies by FFPE introduces DNA fragmentation, hindering analysis of rearrangements. Here the authors introduce FFPE Targeted Locus Capture for identification of translocations in preserved samples.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.15e52e7d12f34aa7b62f485085760e59
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-23695-8