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The role of TNF-αand NFkβin an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine

Authors :
Márcio Alencar Barreira
Márcio Wilker Soares Campelo
Conceição da Silva Martins Rebouças
Ana Paula Bomfim Soares Campelo
Paulo Roberto Leitão de Vasconcelos
Source :
Acta Cirúrgica Brasileira, Vol 38 (2023)
Publication Year :
2023
Publisher :
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2023.

Abstract

ABSTRACT Purpose: To analyze the potential of tumor necrosis factor-α (TNF-α) and factor nuclear kappa B (NF-κB) as colorectal cancer (CRC) biomarkers in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine (1,2-DMH). Methods: Twenty-four male Wistar rats were divided into two groups: sham and 1,2-DMH. First, 1,2-DMH (20 mg/kg/week) was administered for 15 consecutive weeks. In the 25th week, proctocolectomy was conducted. Histopathological analysis, immunohistochemistry, and gene expression of TNF-α and NF-κB were performed. Statistical analysis was performed using GraphPad Prism. The location of aberrant crypt foci (ACF) was analyzed by Kruskal-Wallis’ test. For analyses with two groups with parametric data, the t-test was used; for non-parametric data, the Mann-Whitney’s test was used. P < 0.05 was considered significant. Results: The number of ACF and macroscopic lesions was significantly higher (p < 0.5) in the 1,2-DMH group compared to the sham group, and most ACF were concentrated in the distal segment of the colon. There was a statistically significant increase (p < 0.5) in protein and gene expression of TNF-α and NF-κB in the 1,2-DMH group compared to the sham group. Conclusions: Our results provide supportive evidence that TNF-α and NF-κB pathways are strongly involved in CRC development in rats and might be used as early biomarkers of CRC pathogenesis in experimental studies.

Details

Language :
English
ISSN :
16782674
Volume :
38
Database :
Directory of Open Access Journals
Journal :
Acta Cirúrgica Brasileira
Publication Type :
Academic Journal
Accession number :
edsdoj.14301e9f875d4c888b7d97694f4debd7
Document Type :
article
Full Text :
https://doi.org/10.1590/acb383623