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Effect of carboxyamidotriazole-orotate on proliferation and fatty acid anabolism of human pancreatic cancer cell lines
- Source :
- Jichu yixue yu linchuang, Vol 44, Iss 4, Pp 440-446 (2024)
- Publication Year :
- 2024
- Publisher :
- Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2024.
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Abstract
- Objective To study the effect of carboxyamidotriazole-orotate(CTO) on the proliferation and fatty acid anabolism regulation of human pancreatic cancer cells. Methods Human pancreatic cancer cell lines AsPC-1, AsPC-1/GEM(AR), PANC-1 and MiaPaCa-2 were used as the study subjects; cell survival rate was detected by sulfonylrhodamine B(SRB); the mRNA level of key genes for fatty acid synthesis was detected by qPCR; the protein level of the AMPK/ACC pathway was detected by Western blot; intracellular lipid metabolites were examined by liquid chromatography-mass spectrometry(LC-MS). Results Comparing to control group, CTO significantly decreased the cell viability of AsPC-1, AR, PANC-1, and MiaPaCa-2(P<0.05). CTO down-regulated the mRNA level of key fatty acid synthesis genes(P<0.05). CTO significantly reduced the protein expression of AMPK, ACC and c-Myc(P<0.05), while increasing the protein expression of p-AMPK and p-ACC(P<0.05). CTO decreased lipid metabolite content in AR cells(P<0.05). Conclusions CTO attenuates cellular fatty acid anabolism by inhibition of oncogene c-Myc expression and AMPK/ACC pathway, down-regulates the expression of fatty acid synthesis-related genes, and then inhibits proliferation of the human pancreatic cancer cell lines AsPC-1, AR, PANC-1 and MiaPaCa-2.
Details
- Language :
- Chinese
- ISSN :
- 10016325
- Volume :
- 44
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Jichu yixue yu linchuang
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.1396c3c1c3274e42bf5469d441e53374
- Document Type :
- article
- Full Text :
- https://doi.org/10.16352/j.issn.1001-6325.2024.04.0440