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Effects of the nutrition of different diets and lipid content of the insect host larvae, Galleria mellonella on the efficacy of indigenous entomopathogenic nematodes

Authors :
Mona Ahmed Hussein
Hamdy Abdelnaby Salem
Sayed Hala
Salah Mahmoud
Source :
Journal of Plant Protection Research, Vol vol. 62, Iss No 3, Pp 265-271 (2022)
Publication Year :
2022
Publisher :
Polish Academy of Sciences, 2022.

Abstract

Entomopathogenic nematodes (EPNs) are promising as biocontrol agents for the most economically important insect pest attacking a wide range of host plants. Therefore, the aim of this work was to study the impact of four artificial diets and one natural food on numbers, weights, and total lipid content of the greater wax moth larvae, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) as well as the impact of these diets on the ability of nematode species Heterorhabditis bacteriophora and Steinernema carpocapsae to infect insects and multiply inside an insect host which had been reared on one of five different diets (D1, D2, D3, D4 and D5). The correlation between larvae weight and total lipid content, pathogenicity or multiplication of nematodes was also studied. The obtained results indicated that D2, D5 and D3 gave the highest growth or weights of larvae. The larvae produced weighed 3.71, 3.67 and 3.25 g from 50 g media, respectively. Statistically, larvae weights had a positive and significant correlation with the lipid content in larvae where r = 0.732. On the other hand, infective juveniles (IJs) of nematodes produced from insect hosts reared on D2 and D5 revealed more pathogenicity on larvae, since they caused the highest percent of mortality, 53.33 and 50.0% for H. bacteriophora, and 56.67 and 53.33% for S. carpocapsae, respectively. The total lipid content had a positive and highly significant correlation with the pathogenicity of the two nematode species where r = 0.97 and 0.971, respectively. Ultimately, the supplied foods of the artificial diets D2, D3 and natural beeswax (D5) gave the most suitable chance for developing insect growth and increasing the EPN quality and enhancing the potential of EPNs as biological control agents against different insect pests.

Details

Language :
English
ISSN :
14274345
Volume :
. 62
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Journal of Plant Protection Research
Publication Type :
Academic Journal
Accession number :
edsdoj.130a24a5a114e86bd846e70f3f045f7
Document Type :
article
Full Text :
https://doi.org/10.24425/jppr.2022.142133