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Porcine Reproductive and Respiratory Syndrome Virus Engineered by Serine Substitution on the 44th Amino Acid of GP5 Resulted in a Potential Vaccine Candidate with the Ability to Produce High Levels of Neutralizing Antibody

Authors :
Jong-Chul Choi
Min-Sik Kim
Hwi-Yeon Choi
Yeong-Lim Kang
In-Yeong Choi
Sung-Won Jung
Ji-Yun Jeong
Min-Chul Kim
Andrew Y. Cho
Ji-Ho Lee
Dong-Hun Lee
Sang-Won Lee
Seung-Yong Park
Chang-Seon Song
In-Soo Choi
Joong-Bok Lee
Source :
Veterinary Sciences, Vol 10, Iss 3, p 191 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

N-linked glycans covering GP5 neutralizing epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) have been proposed to act as a sheath blocking the production of neutralizing antibodies. Herein, we genetically engineered PRRSV with serine (S) substitution on the 44th asparagine (N) on the GP5 ectodomain of PRRSV-2 lineage-1. To evaluate the recombinant PRRSV, in vivo experiments were performed in piglets. The recombinant virus group showed no viremia until 42 days post-inoculation (dpi), and the rectal temperature and average daily weight gain were in the normal range at the same time point as the negative control group. On the 42 dpi, both groups were challenged with the wild-type virus. The recombinant PRRSV group showed lower rectal temperature, viremia, and the lung lesions than that of the negative control group for 19 days post-challenge (dpc). Additionally, the recombinant virus induced 4.50 ± 3.00 (log2) and 8.25 ± 0.96 (log2) of neutralizing antibody before and after challenge, respectively. Taken together, this study confirmed that N44S substitution can create an infectious PRRSV that strongly induces neutralizing antibodies. In addition, the vCSL1-GP5-N44S mutant that we produced was confirmed to have potential as a vaccine candidate, showing good safety and protective effects in pigs.

Details

Language :
English
ISSN :
23067381
Volume :
10
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Veterinary Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.12fd423fa44f4ed881b0412a6666d5fc
Document Type :
article
Full Text :
https://doi.org/10.3390/vetsci10030191