Back to Search
Start Over
MDR1 is Related to Intestinal Epithelial Injury Induced by Acetylsalicylic Acid
- Source :
- Cellular Physiology and Biochemistry, Vol 32, Iss 4, Pp 942-950 (2013)
- Publication Year :
- 2013
- Publisher :
- Cell Physiol Biochem Press GmbH & Co KG, 2013.
-
Abstract
- Background/Aims: Although the cytotoxicity of aspirin against the intestinal epithelium is a major clinical problem, little is known about its pathogenesis. We assessed the involvement of Multi Drug Resistance (MDR) 1 in intestinal epithelial cell injury caused by aspirin using MDR1 gene-transfected Caco2 cells. Methods: Caco2 cells were treated with various concentrations of aspirin for 24 h. After treatment of Caco2 cells with verapamil, a specific inhibitor of MDR1, we assessed the extent of cell injury using a WST-8 assay at 24 h after aspirin-stimulation. We performed the same procedure in MDR1 gene-transfected Caco2 cells. To determine the function of MDR1 in the metabolism of aspirin, flux study was performed using 14C-labeled aspirin. Results: The level of aspirin-induced cell injury was higher in verapamil-treated Caco2 cells than in control cells and was less serious in MDR1-transfected Caco2 cells than in control vector-transfected cells. The efflux of 14C-labeled aspirin was higher in verapamil-treated Caco2 cells than in control cells. Conclusion: These data suggest that aspirin effux occurs through the MDR1 transporter and that the MDR1 transporter is involved in the pathogenesis of aspirin-induced cell injury.
- Subjects :
- MDR1
Aspirin
Intestinal epithelial injury
Physiology
QP1-981
Biochemistry
QD415-436
Subjects
Details
- Language :
- English
- ISSN :
- 10158987 and 14219778
- Volume :
- 32
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Cellular Physiology and Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.12860c168a27475d8c128156f9a4553c
- Document Type :
- article
- Full Text :
- https://doi.org/10.1159/000354497