Back to Search
Start Over
Role of Nicotinic Acetylcholine Receptor α3 and α7 Subunits in Detrusor Overactivity Induced by Partial Bladder Outlet Obstruction in Rats
- Source :
- International Neurourology Journal, Vol 19, Iss 1, Pp 12-18 (2015)
- Publication Year :
- 2015
- Publisher :
- Korean Continence Society, 2015.
-
Abstract
- Purpose: To investigate the role of α3 and α7 nicotinic acetylcholine receptor subunits (nAChRs) in the bladder, using a rat model with detrusor overactivity induced by partial bladder outlet obstruction (BOO). Methods: Forty Sprague-Dawley rats were used: 10 were sham-operated (control group) and 30 were observed for 3 weeks after partial BOO. BOO-induced rats were further divided into 3 groups: Two groups of 10 rats each received intravesicular infusions with hexamethonium (HM group; n=10) or methyllycaconitine (MLC group; n=10), which are antagonists for α3 and α7 nAChRs, respectively. The remaining BOO-induced rats received only saline infusion (BOO group; n=10). Based on the contraction interval measurements using cystometrogram, the contraction pressure and nonvoiding bladder contractions were compared between the control and the three BOO-induced groups. Immunofluorescent staining and Western blotting were used to analyze α3 and α7 nAChRs levels. Results: The contraction interval of the MLC group was higher than that of the BOO group (P
Details
- Language :
- English
- ISSN :
- 20934777 and 20936931
- Volume :
- 19
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- International Neurourology Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.11a10510d6ee46c4a3e0baa589a0e630
- Document Type :
- article
- Full Text :
- https://doi.org/10.5213/inj.2015.19.1.12