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Select Whole-Cell Biofilm-Based Immunogens Protect against a Virulent Staphylococcus Isolate in a Stringent Implant Model of Infection

Authors :
Stephen J. Dollery
Janette M. Harro
Taralyn J. Wiggins
Brendan P. Wille
Peter C. Kim
John K. Tobin
Ruth V. Bushnell
Naomi J. P. E. R. Tasker
David A. MacLeod
Gregory J. Tobin
Source :
Vaccines, Vol 10, Iss 6, p 833 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Many microbes of concern to human health remain without vaccines. We have developed a whole-microbe inactivation technology that enables us to rapidly inactivate large quantities of a pathogen while retaining epitopes that were destroyed by previous inactivation methods. The method that we call UVC-MDP inactivation can be used to make whole-cell vaccines with increased potency. We and others are exploring the possibility of using improved irradiation-inactivation technologies to develop whole-cell vaccines for numerous antibiotic-resistant microbes. Here, we apply UVC-MDP to produce candidate MRSA vaccines which we test in a stringent tibia implant model of infection challenged with a virulent MSRA strain. We report high levels of clearance in the model and observe a pattern of protection that correlates with the immunogen protein profile used for vaccination.

Details

Language :
English
ISSN :
2076393X
Volume :
10
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Vaccines
Publication Type :
Academic Journal
Accession number :
edsdoj.10dfb6456f24fdfb13f1dc0daf63ee1
Document Type :
article
Full Text :
https://doi.org/10.3390/vaccines10060833