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VvERF111 Regulates Chlorophyll Degradation by Activating Expression of VvCLH1, Leading to Rachis Browning in Grape

Authors :
Dongfang Zou
Jingwen Li
Xia Ye
Xianbo Zheng
Bin Tan
Jun Cheng
Wei Wang
Zhiqian Li
Jiancan Feng
Source :
Horticulturae, Vol 9, Iss 4, p 438 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

The plant growth regulator ethylene influences rachis browning in grape (Vitis vinifera L.). Although the ethylene signaling pathway is well defined, there is limited knowledge on its mode of action during rachis browning. Here, we show that an ethylene response factor (VvERF111) positively regulates chlorophyll degradation in rachis by binding to a DRE motif in the promoter of VvCLH1. The expression of VvERF111 and VvCLH1 in rachis was induced by ethylene and inhibited by 1-methylcyclopropene (1-MCP). VvERF111 belongs to the ERF IX subfamily of the APETALA2/ethylene responsive factor (AP2/ERF) superfamily, shows transcriptional activity in yeast, and is localized in the nucleus and membrane. The transient overexpression of VvERF111 or chlorophyllase (VvCLH1) in grape leaves accelerated chlorophyll degradation. In VvERF111-overexpressing leaves, transcript levels of VvCLH1 were also increased. Our findings offer a deeper understanding of the transcriptional regulation of chlorophyll degradation during the rachis browning of grape.

Details

Language :
English
ISSN :
23117524
Volume :
9
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Horticulturae
Publication Type :
Academic Journal
Accession number :
edsdoj.0f0e7fd1a4c4445c8d1cf6af7e726a53
Document Type :
article
Full Text :
https://doi.org/10.3390/horticulturae9040438