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Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue

Authors :
Eriko Abe
Takashi J. Fuwa
Kyoka Hoshi
Takashi Saito
Takenobu Murakami
Masakazu Miyajima
Norihiro Ogawa
Hiroyasu Akatsu
Yoshio Hashizume
Yasuhiro Hashimoto
Takashi Honda
Source :
Metabolites, Vol 12, Iss 7, p 594 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Iron is an essential nutrient in the body. However, iron generates oxidative stress and hence needs to be bound to carrier proteins such as the glycoprotein transferrin (Tf) in body fluids. We previously reported that cerebrospinal fluid contains Tf glycan-isoforms that are derived from the brain, but their origins at the cellular level in the brain have not yet been elucidated. In the present report, we described the localization of Tf protein and mRNA in mouse and human brain tissue. In situ hybridization of mouse brain tissue revealed that Tf mRNA is expressed by different cell types such as epithelial cells in the choroid plexus, oligodendrocyte-like cells in the medulla, and neurons in the cortex, hippocampus, and basal ganglia. In contrast, Tf protein was barely detected by immunohistochemistry in hippocampal and some cortical neurons, but it was detected in other types of cells such as oligodendrocyte-like cells and choroid plexus epithelial cells. The results showed that Tf mRNA is expressed by neural cells, while Tf protein is expressed in different brain regions, though at very low levels in hippocampal neurons. Low Tf level in the hippocampus may increases susceptibility to iron-induced oxidative stress, and account for neuron death in neurodegenerative diseases.

Details

Language :
English
ISSN :
22181989
Volume :
12
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Metabolites
Publication Type :
Academic Journal
Accession number :
edsdoj.0f0717c9ea1d4a13b3d98d1c67ce6dc0
Document Type :
article
Full Text :
https://doi.org/10.3390/metabo12070594