In-vitro Susceptibility of Levofloxacin against Different Clinical Isolates

Background: Levofloxacin is a broad spectrum quinolone, widely used to treat infections caused by gram negative and gram positive bacteria. Development of resistance by pathogens against different broadspectrum antibiotics is increasing and now becoming a global issue. Objectives: The aim of the study is to evaluate the current sensitivity pattern of levofloxacin against various common clinical isolates like Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa,and Klebsiellapneumoniae. Methods: A total of one hundred and ten samples were collected from different pathological laboratories of Karachi, Pakistan. The above mentioned pathogens were isolated from blood, stool/urine, sputum, skin samples. Results: Results show least resistance of levofloxacin against E.coli (27.5%), and P.aeruginosa (27%), while S.aureus possessed highest resistance (45%). Conclusion: Study concluded levofloxacin still possesses excellent anti-microbial activity against common pathogens. Routine monitoring and surveillance is further required to ensure effective treatment regimens to community. KEY WORDS: Levofloxacin, Resistance, Susceptibility, Clinical Isolates, Pathogens.