Back to Search Start Over

Current Challenges in Providing Good Leukapheresis Products for Manufacturing of CAR-T Cells for Patients with Relapsed/Refractory NHL or ALL

Authors :
Felix Korell
Sascha Laier
Sandra Sauer
Kaya Veelken
Hannah Hennemann
Maria-Luisa Schubert
Tim Sauer
Petra Pavel
Carsten Mueller-Tidow
Peter Dreger
Michael Schmitt
Anita Schmitt
Source :
Cells, Vol 9, Iss 5, p 1225 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Background: T lymphocyte collection through leukapheresis is an essential step for chimeric antigen receptor T (CAR-T) cell therapy. Timing of apheresis is challenging in heavily pretreated patients who suffer from rapid progressive disease and receive T cell impairing medication. Methods: A total of 75 unstimulated leukaphereses were analyzed including 45 aphereses in patients and 30 in healthy donors. Thereof, 41 adult patients with Non-Hodgkin’s lymphoma (85%) or acute lymphoblastic leukemia (15%) underwent leukapheresis for CAR-T cell production. Results: Sufficient lymphocytes were harvested from all patients even from those with low peripheral lymphocyte counts of 0.18/nL. Only four patients required a second leukapheresis session. Leukapheresis products contained a median of 98 × 108 (9 - 341 × 108) total nucleated cells (TNC) with 38 × 108 (4 - 232 × 108) CD3+ T cells. Leukapheresis products from healthy donors as well as from patients in complete remission were characterized by high TNC and CD3+ T lymphocyte counts. CAR-T cell products could be manufactured for all but one patient. Conclusions: Sufficient yield of lymphocytes for CAR-T cell production is feasible also for patients with low peripheral blood counts. Up to 12–15 L blood volume should be processed in patients with absolute lymphocyte counts ≤ 1.0/nL.

Details

Language :
English
ISSN :
20734409
Volume :
9
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
edsdoj.0e8b82aa10024efab83f76309a00d0f7
Document Type :
article
Full Text :
https://doi.org/10.3390/cells9051225