Back to Search
Start Over
Method Optimization: Analysis of Benzbromarone and Tolfenamic Acid in Citrus Tissues and Soil Using Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry
- Source :
- Frontiers in Plant Science, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Herein, an analytical method was developed for extraction and quantification of benzbromarone and tolfenamic acid in citrus and soil matrices using liquid-liquid extraction followed by liquid chromatography-triple quadrupole-tandem mass spectrometry analysis. The compounds were extracted using 0.1% formic acid in 6:4 ethyl acetate and n-hexane solution, and the analytes were separated using a mixture of 0.1% formic acid in ultrapure water and 0.1% formic acid in acetonitrile as mobile phase. A six-point in-matrix calibration curve was constructed providing good linearity with coefficients of determination R2 ≥ 0.98. The limits of detection and quantification for benzbromarone and tolfenamic acid were 3.0 and 10.0 μg/kg in roots, peel, juice, and soil, and 4.0 and 12.0 μg/kg for leaves samples, respectively. The method yielded excellent recoveries between 81.3 and 101.2%, with relative standard deviation ≤9.5% in the matrices. The developed technique provides a simple and sensitive method for the determination of the chemicals and can be applied to agricultural practices.
Details
- Language :
- English
- ISSN :
- 1664462X and 57894418
- Volume :
- 11
- Database :
- Directory of Open Access Journals
- Journal :
- Frontiers in Plant Science
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0e1efe1f5789441885b41d09381b66e6
- Document Type :
- article
- Full Text :
- https://doi.org/10.3389/fpls.2020.00222