Detection of WNT7A Methylation in Peripheral Blood of NSCLC Patients and Its Diagnostic Value

Objective To explore the relation between WNT7A methylation and clinical factors of NSCLC patients. Methods We selected 120 NSCLC patients as NSCLC group and 120 healthy people as control group. The detection rate of WNT7A methylation in two groups was compared by methylation specific polymerase chain reaction, and the relation between WNT7A methylation and clinical factors of NSCLC patients was compared. Results The methylation rate of WNT7A in peripheral blood of NSCLC group was significantly higher than that in control group (P < 0.05). In peripheral blood of NSCLC group, there was no significant correlation between WNT7A gene methylation and gender, pathological type, age, smoking history(P > 0.05). The methylation rate of WNT7A gene was significantly correlated with tumor size, distant metastasis, TNM stage and pleural effusion(P < 0.05). The detection rate of WNT7A gene methylation in peripheral blood of NSCLC patients with pleural effusion, tumor size > 5cm and TNM stage Ⅲ-Ⅳ was significantly higher than that of patients without pleural effusion, tumor size ≤5cm and stage Ⅰ-Ⅱ (P < 0.05). Conclusion The detection rate of WNT7A gene methylation is high in NSCLC patients, which can be used as an effective diagnostic basis for NSCLC.