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Invited Article: Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces

Authors :
Wolfgang Langbein
David Regan
Iestyn Pope
Paola Borri
Source :
APL Photonics, Vol 3, Iss 9, Pp 092402-092402-18 (2018)
Publication Year :
2018
Publisher :
AIP Publishing LLC, 2018.

Abstract

Coherent Raman Scattering (CRS) has emerged in the last decade as a powerful multiphoton microscopy technique offering chemically specific label-free imaging in real time with high three-dimensional spatial resolution. Many technical realizations of CRS microscopy have been proposed to remove, suppress, or account for the non-resonant background in the nonlinear susceptibility which complicates spectral analysis and reduces image contrast. Here, we demonstrate coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epi-geometry (eH-CARS), providing background-free chemically specific image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. We show the sensitivity and selectivity of eH-CARS in comparison with forward CARS and stimulated Raman scattering on polystyrene beads in agarose gel. As an important biologically relevant application, we demonstrate eH-CARS imaging of individual lipid bilayers with high contrast and topographic sensitivity.

Details

Language :
English
ISSN :
23780967
Volume :
3
Issue :
9
Database :
Directory of Open Access Journals
Journal :
APL Photonics
Publication Type :
Academic Journal
Accession number :
edsdoj.0d6ff8791fa84e91be98ebaa92d98c54
Document Type :
article
Full Text :
https://doi.org/10.1063/1.5027256