Back to Search
Start Over
Invited Article: Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces
- Source :
- APL Photonics, Vol 3, Iss 9, Pp 092402-092402-18 (2018)
- Publication Year :
- 2018
- Publisher :
- AIP Publishing LLC, 2018.
-
Abstract
- Coherent Raman Scattering (CRS) has emerged in the last decade as a powerful multiphoton microscopy technique offering chemically specific label-free imaging in real time with high three-dimensional spatial resolution. Many technical realizations of CRS microscopy have been proposed to remove, suppress, or account for the non-resonant background in the nonlinear susceptibility which complicates spectral analysis and reduces image contrast. Here, we demonstrate coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epi-geometry (eH-CARS), providing background-free chemically specific image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. We show the sensitivity and selectivity of eH-CARS in comparison with forward CARS and stimulated Raman scattering on polystyrene beads in agarose gel. As an important biologically relevant application, we demonstrate eH-CARS imaging of individual lipid bilayers with high contrast and topographic sensitivity.
- Subjects :
- Applied optics. Photonics
TA1501-1820
Subjects
Details
- Language :
- English
- ISSN :
- 23780967
- Volume :
- 3
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- APL Photonics
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0d6ff8791fa84e91be98ebaa92d98c54
- Document Type :
- article
- Full Text :
- https://doi.org/10.1063/1.5027256