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Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.)

Authors :
Yujia Leng
Tao Tao
Shuai Lu
Ran Liu
Qingqing Yang
Mingqiu Zhang
Lianmin Hong
Qianqian Guo
Xinzhe Ren
Zhidi Yang
Xiuling Cai
Sukui Jin
Jiping Gao
Source :
Agriculture, Vol 14, Iss 7, p 1002 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The tiller angle, which is an important agronomic trait, determines plant architecture and greatly influences the grain yield of rice. In this study, a population of chromosome segment substitution lines derived from a cross between a japonica variety with a compact plant architecture—Koshihikari—and an indica variety with a spread-out plant architecture—Nona Bokra—was used to investigate the genetic basis of the tiller angle. Five quantitative trait loci (qTA1, qTA5, qTA9-1, qTA9-2, and qTA11) for the tiller angle were detected on chromosomes 1, 5, 9, 9, and 11 in two different environments. The phenotypic variation in these QTLs ranged from 3.78% to 8.22%. Two pairs of digenic epistatic QTLs were detected in Lingshui. The epistatic interaction explained 15.19% and 13.60% of the phenotypic variance, respectively. Among the five QTLs, qTA9-2 was detected in both environments. An F2 mapping population containing the qTA9-2 QTL was established. The location of qTA9-2 was narrowed down to a 187 kb region between InDel markers M9 and M10 on chromosome 9. Thirty open reading frames (ORFs), including TAC1, a gene known to regulate the tiller angle, were identified in this region. The gene sequencing results suggested that a base substitution from G to A at position 1557 in the 3′-untranslated region led to a difference in the expression of qTA9-2 in Koshihikari and Nona Bokra. These findings provide a potential gene resource for the improvement of rice plant architecture.

Details

Language :
English
ISSN :
14071002 and 20770472
Volume :
14
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Agriculture
Publication Type :
Academic Journal
Accession number :
edsdoj.0cb502c64a2645298e88885cd2afbcc5
Document Type :
article
Full Text :
https://doi.org/10.3390/agriculture14071002