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SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease
- Source :
- Biological Procedures Online, Vol 11, Iss 1, p 9007 (2009)
- Publication Year :
- 2009
- Publisher :
- BMC, 2009.
-
Abstract
- Abstract Kidney stone disease is a common health problem in industrialised nations. We developed a SDS-PAGE-based method to quantify Tamm Horsfall glycoprotein (THP) for screening of kidney stone disease. Urinary proteins were extracted by using ammonium sulphate precipitation at 0.27 g salt/mL urine. The resulted pellet was dissolved in TSE buffer. Ten microliters of the urinary proteins extract was loaded and separated on 10% SDS-PAGE under reducing condition. THP migrated as single band in SDS-PAGE. The assay reproducibility and repeatability were 4.8% CV and 2.6% CV, respectively. A total of 117 healthy subjects and 58 stone patients were tested using this assay, and a distinct cut-off (P < 0.05) at 5.6 μg/mL THP concentration was used to distinguish stone patients from healthy subjects. The sensitivity and specificity of the method were 92.3% and 83.3%, respectively.
Details
- Language :
- English
- ISSN :
- 14809222
- Volume :
- 11
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Biological Procedures Online
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0c7217d39d34e11852e61c241e7dcc2
- Document Type :
- article
- Full Text :
- https://doi.org/10.1007/s12575-009-9007-y