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Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion

Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion

Authors :
Cinzia Di Franco
Eleonora Macchia
Michele Catacchio
Mariapia Caputo
Cecilia Scandurra
Lucia Sarcina
Paolo Bollella
Angelo Tricase
Massimo Innocenti
Riccardo Funari
Matteo Piscitelli
Gaetano Scamarcio
Luisa Torsi
Source :
Advanced Science, Vol 12, Iss 1, Pp n/a-n/a (2025)
Publication Year :
2025
Publisher :
Wiley, 2025.

Abstract

Abstract The electric dipoles of proteins in a biolayer determine their dielectric properties through the polarization density P. Hence, its reproducibility is crucial for applications, particularly in bioelectronics. Biolayers encompassing capturing antibodies covalently bound at a biosensing interface are generally preferred for their assumed higher stability. However, surface physisorption is shown to offer advantages like easily scalable fabrication processes and high stability. The present study investigates the effects of electric‐field (EF)‐cycling of anti‐Immunoglobulin M (anti‐IgM) biolayers physisorbed on Au. The impact of EF‐cycling on the dielectric, optical, and mechanical properties of anti‐IgM biolayer is investigated. A reduction of the dispersion (standard deviation over a set of 31 samples) of the measured P values is observed, while the set median stays almost constant. Hence, physisorption combined with EF cycling, results in a biolayer with highly reproducible bioelectronic properties. Additionally, the study provides important insights into the mechanisms of dielectric rearrangement of dipole moments in capturing biolayers after EF‐cycling. Notably, EF‐cycling acts as an annealing process, driving the proteins in the biolayer into a statistically more probable and stable conformational state. Understanding these phenomena enhances the knowledge of the properties of physisorbed biolayers and can inform design strategies for bioelectronic devices.

Details

Language :
English
ISSN :
21983844
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Advanced Science
Publication Type :
Academic Journal
Accession number :
edsdoj.0c3dfb0315124d689b1c615f2f9572fb
Document Type :
article
Full Text :
https://doi.org/10.1002/advs.202412347