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New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
- Source :
- PPAR Research, Vol 2017 (2017)
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ’s potential ligands.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 16874757 and 16874765
- Volume :
- 2017
- Database :
- Directory of Open Access Journals
- Journal :
- PPAR Research
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0c2131e3e6aa465d9e507321730b87bb
- Document Type :
- article
- Full Text :
- https://doi.org/10.1155/2017/6139107