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Harvesting the Power of Green Synthesis: Gold Nanoparticles Tailored for Prostate Cancer Therapy

Authors :
Marco Oliveira
André Sousa
Sara Sá
Sílvia Soares
Ana Cláudia Pereira
Ana Catarina Rocha
Patrick Pais
Diogo Ferreira
Cátia Almeida
Carla Luís
Cláudio Lima
Fábio Almeida
Álvaro Gestoso
Miguel-Correa Duarte
Pedro Barata
Daniela Martins-Mendes
Pilar Baylina
Carla F. Pereira
Rúben Fernandes
Source :
International Journal of Molecular Sciences, Vol 25, Iss 4, p 2277 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Biosynthetic gold nanoparticles (bAuNPs) present a promising avenue for enhancing bio-compatibility and offering an economically and environmentally responsible alternative to traditional production methods, achieved through a reduction in the use of hazardous chemicals. While the potential of bAuNPs as anticancer agents has been explored, there is a limited body of research focusing on the crucial physicochemical conditions influencing bAuNP production. In this study, we aim to identify the optimal growth phase of Pseudomonas aeruginosa cultures that maximizes the redox potential and coordinates the formation of bAuNPs with increased efficiency. The investigation employs 2,6-dichlorophenolindophenol (DCIP) as a redox indicator. Simultaneously, we explore the impact of temperature, pH, and incubation duration on the biosynthesis of bAuNPs, with a specific emphasis on their potential application as antitumor agents. Characterization of the resulting bAuNPs is conducted using ATR-FT-IR, TEM, and UV-Vis spectroscopy. To gain insights into the anticancer potential of bAuNPs, an experimental model is employed, utilizing both non-neoplastic (HPEpiC) and neoplastic (PC3) epithelial cell lines. Notably, P. aeruginosa cultures at 9 h/OD600 = 1, combined with biosynthesis at pH 9.0 for 24 h at 58 °C, produce bAuNPs that exhibit smaller, more spherical, and less aggregated characteristics. Crucially, these nanoparticles demonstrate negligible effects on HPEpiC cells while significantly impacting PC3 cells, resulting in reduced viability, migration, and lower IL-6 levels. This research lays the groundwork for the development of more specialized, economical, and ecologically friendly treatment modalities.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
4
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.0a85636a76774a07a9bb2435c9141e9c
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25042277