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Candidate genes identification and RNA-seq based pathway analysis associated with primary angle-closure glaucoma with cataract

Authors :
Min Liu
Fei Hu
Caifeng Lei
Min Fu
Xue Li
Ling Yu
Source :
BMC Ophthalmology, Vol 23, Iss 1, Pp 1-9 (2023)
Publication Year :
2023
Publisher :
BMC, 2023.

Abstract

Abstract Background Cataract is commonly observed in patients with primary angle-closure glaucoma; however, its underlying pathological mechanisms remain unclear. This study aimed to improve our knowledge on the pathological processes involved in primary angle-closure glaucoma (PACG) by identifying potential prognostic genes associated with cataract progression. Methods Thirty anterior capsular membrane samples were collected from PACG patients with cataracts and age-related cataracts. Differentially expressed genes (DEGs) between these two cohorts were analyzed using high-throughput sequencing. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to screen the DEGs, and potential prognostic markers and their coexpression network were then predicted by bioinformatic analyses. The DEGs were further validated by reverse transcription-quantitative polymerase chain reaction. Results A total of 399 DEGs were found to be specifically associated with cataracts development in PACG patients, among which 177 and 221 DEGs were upregulated and downregulated, respectively. STRING and Cytoscape network analyses revealed seven genes—CTGF, FOS, CAV1, CYR61, ICAM1, EGR1, and NR4A1—that were remarkably enriched and mainly involved in the MAPK, PI3K/Akt, Toll-like receptor, and TNF signaling pathways. RT-qPCR-based validation further confirmed that the sequencing results were accurate and reliable. Conclusions Herein, we identified seven genes and their signaling pathways that may contribute to cataract progression in patients with high intraocular pressure. Taken together, our findings highlight new molecular mechanisms that may explain the high incidence of cataracts in PACG patients. In addition, the genes identified herein may represent new foundations for the development of therapeutic strategies for PACG with cataract.

Details

Language :
English
ISSN :
14712415
Volume :
23
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Ophthalmology
Publication Type :
Academic Journal
Accession number :
edsdoj.0a7b0c170da9447f93a7b2da981522ba
Document Type :
article
Full Text :
https://doi.org/10.1186/s12886-023-02950-0