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Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I

Authors :
Afonso S. I. Salgado
Juliana Stramosk
Daniela D. Ludtke
Ana C. C. Kuci
Daiana C. Salm
Lisandro A. Ceci
Fabricia Petronilho
Drielly Florentino
Lucineia G. Danielski
Aline Gassenferth
Luana R. Souza
Gislaine T. Rezin
Adair R. S. Santos
Leidiane Mazzardo-Martins
William R. Reed
Daniel F. Martins
Source :
Brain Sciences, Vol 9, Iss 8, p 197 (2019)
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

Complex regional pain syndrome type I (CRPS-I) is a chronic painful condition. We investigated whether manual therapy (MT), in a chronic post-ischemia pain (CPIP) model, is capable of reducing pain behavior and oxidative stress. Male Swiss mice were subjected to ischemia-reperfusion (IR) to mimic CRPS-I. Animals received ankle joint mobilization 48h after the IR procedure, and response to mechanical stimuli was evaluated. For biochemical analyses, mitochondrial function as well as oxidative stress thiobarbituric acid reactive substances (TBARS), protein carbonyls, antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) levels were determined. IR induced mechanical hyperalgesia which was subsequently reduced by acute MT treatment. The concentrations of oxidative stress parameters were increased following IR with MT treatment preventing these increases in malondialdehyde (MDA) and carbonyls protein. IR diminished the levels of SOD and CAT activity and MT treatment prevented this decrease in CAT but not in SOD activity. IR also diminished mitochondrial complex activity, and MT treatment was ineffective in preventing this decrease. In conclusion, repeated sessions of MT resulted in antihyperalgesic effects mediated, at least partially, through the prevention of an increase of MDA and protein carbonyls levels and an improvement in the antioxidant defense system.

Details

Language :
English
ISSN :
20763425
Volume :
9
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Brain Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.0a226844c654e668c86e055a7690236
Document Type :
article
Full Text :
https://doi.org/10.3390/brainsci9080197