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PDA-mediated Mild Photothermal Therapy Combined with Autophagy Inhibitors Kill Breast Cancer Cells
- Source :
- Zhongliu Fangzhi Yanjiu, Vol 48, Iss 7, Pp 659-666 (2021)
- Publication Year :
- 2021
- Publisher :
- Magazine House of Cancer Research on Prevention and Treatment, 2021.
-
Abstract
- Objective To explore whether inhibiting autophagy can enhance the sensitivity of photothermal treatment under mild photothermal conditions. Methods CQ@PLGA@PDA NPs were prepared by an improved double emulsification method and a PDA-based surface modification method. After basic characterization, CCK-8 method was used to detect the cytotoxicity of nanoparticles; the near-infrared laser irradiation nanoparticle solution was used to detect the heating effect; CCK-8 method and live-dead cell staining were used to detect the killing effect of tumor cells; Western blot was used to detect the expression of autophagy-related proteins. Results The CQ@PLGA@PDA NPs were successfully prepared, with a particle size of 253.10±2.39 nm, a zeta potential of -22.57±0.80 mV, uniform particle size and good dispersion. The temperature of nanoparticle solution increased to 45℃ after the near-infrared laser irradiation for 10 min. CQ@PLGA@PDA NPs had no obvious toxicity to cells. The survival rates of breast cancer cell MDA-MB-231 and mouse embryonic fibroblast NIH-3T3 cell were above 95%. The inhibition of autophagy under mild photothermal conditions could improve the sensitivity of photothermal therapy. Conclusion The prepared CQ@PLGA@PDA NPs have good photothermal performance and high biological safety; by inhibiting autophagy, they can effectively kill tumor cells under mild photothermal conditions(< 50℃).
Details
- Language :
- Chinese
- ISSN :
- 10008578
- Volume :
- 48
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Zhongliu Fangzhi Yanjiu
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.09737c1a3c204769b5501aa5e60ca0c9
- Document Type :
- article
- Full Text :
- https://doi.org/10.3971/j.issn.1000-8578.2021.21.0142