Back to Search
Start Over
Quantitative RT-PCR: Pitfalls and Potential
- Source :
- BioTechniques, Vol 26, Iss 1, Pp 112-125 (1999)
- Publication Year :
- 1999
- Publisher :
- Future Science Ltd, 1999.
-
Abstract
- Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR involves correction for experimental variations in individual RT and PCR efficiencies. This review addresses the mathematics of RT-PCR, choice of RNA standards (internal vs. external) and quantification strategies (competitive, noncompetitive and kinetic [real-time] amplification). Finally, the discussion turns to practical considerations in experimental design. It is hoped that this review will be appropriate for those undertaking these experiments for the first time or wishing to improve (or validate) a technique in what is frequently a confusing and contradictory field.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 19409818 and 07366205
- Volume :
- 26
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- BioTechniques
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0963bfbe255c42178912b9848380a90d
- Document Type :
- article
- Full Text :
- https://doi.org/10.2144/99261rv01