The Association between Polymorphism of Xrcc3 Gene and Colorectal Cancer: A Case-Control Study

Background: Colorectal cancer is the third cancer which results in death in western countries. Some of the risk factors of this cancer are age, inadequate diet, obesity, inactivity, genetic changes, etc. Considering the relationship between genetic changes with colorectal cancer is the subject of recent studies, and one of the subjects which have been focused is T241M polymorphism in Xrcc3 gene. Methods: In this case-control study, 90 patients with colorectal cancer and 83 healthy people were included. Genomic DNA was extracted from peripheral blood, and genotype distribution in the region of polymorphism was studied. By designing of primers, the considered area was amplified and genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) method. Findings: We observed the association between T241M polymorphism and colorectal cancer. Besides, there was a meaningful relationship between aging and family history with this cancer. On the other hand, smoking was not associated with colon cancer, but it also showed a significant role in the incidence and metastasis of rectal cancer. Conclusion: The relationship between T241M polymorphism and increased risk of colorectal cancer, along with family history of cancer, old age, and smoking were reported as risk factors for colorectal cancer. Therefore, the results of this research can show the need for further investigation in subsequent studies.