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Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin

Authors :
Đokić Divna Đ.
Janković Drina L.
Maksin Tatjana N.
Source :
Journal of the Serbian Chemical Society, Vol 67, Iss 8-9, Pp 573-579 (2002)
Publication Year :
2002
Publisher :
Serbian Chemical Society, 2002.

Abstract

Anew procedure for the aggregation of human albumin and 99mTc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99mTc-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman No 1 and ITLC-SAstrips. Asystem of two solvents (acetone and 1MNaCl or 0.9%NaCl) was used for 3MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency (> 98.5%) and percent radiochemical impurities of 99mTc-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000 –350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes.

Details

Language :
English
ISSN :
03525139 and 18207421
Volume :
67
Issue :
8-9
Database :
Directory of Open Access Journals
Journal :
Journal of the Serbian Chemical Society
Publication Type :
Academic Journal
Accession number :
edsdoj.075e7c9bb808451f874bfa5ddba10fa9
Document Type :
article
Full Text :
https://doi.org/10.2298/JSC0209573D