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Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin
- Source :
- Journal of the Serbian Chemical Society, Vol 67, Iss 8-9, Pp 573-579 (2002)
- Publication Year :
- 2002
- Publisher :
- Serbian Chemical Society, 2002.
-
Abstract
- Anew procedure for the aggregation of human albumin and 99mTc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99mTc-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman No 1 and ITLC-SAstrips. Asystem of two solvents (acetone and 1MNaCl or 0.9%NaCl) was used for 3MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency (> 98.5%) and percent radiochemical impurities of 99mTc-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000 –350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes.
- Subjects :
- human albumin macroaggregates
99mtc-labelling
quality control.
Chemistry
QD1-999
Subjects
Details
- Language :
- English
- ISSN :
- 03525139 and 18207421
- Volume :
- 67
- Issue :
- 8-9
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of the Serbian Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.075e7c9bb808451f874bfa5ddba10fa9
- Document Type :
- article
- Full Text :
- https://doi.org/10.2298/JSC0209573D