Biosynthesis of Copper Oxide Nanoparticles with Potential Biomedical Applications

Navid Rabiee,1 Mojtaba Bagherzadeh,1 Mahsa Kiani,1 Amir Mohammad Ghadiri,2 Fatemeh Etessamifar,1 Amir Hossein Jaberizadeh,2 Alireza Shakeri2 1Department of Chemistry, Sharif University of Technology, Tehran 11155-3516, Iran; 2School of Chemistry, College of Science, University of Tehran, Tehran, IranCorrespondence: Mojtaba Bagherzadeh Tel +98(21)66165301Email bagherzadeh@sharif.eduIntroduction: In recent years, the use of cost-effective, multifunctional, environmentally friendly and simple prepared nanomaterials/nanoparticles have been emerged considerably. In this manner, different synthesizing methods were reported and optimized, but there is still lack of a comprehensive method with multifunctional properties.Materials and Methods: In this study, we aim to synthesis the copper oxide nanoparticles using Achillea millefolium leaf extracts for the first time. Catalytic activity was investigated by in situ azide alkyne cycloaddition click and also A3 coupling reaction, and optimized in terms of temperature, solvent, and time of the reaction. Furthermore, the photocatalytic activity of the synthesized nanoparticles was screened in terms of degradation methylene blue dye. Biological activity of the synthesized nanoparticles was evaluated in terms of antibacterial and anti-fungal assessments against Staphylococcus aureus, M. tuberculosis, E. coli, K. pneumoniae, P. mirabili, C. diphtheriae and S. pyogenes bacteria’s and G. albicans, A. flavus, M. canis and G. glabrata fungus. In the next step, the biosynthesized CuO-NPs were screened by MTT and NTU assays.Results: Based on our knowledge, this is a comprehensive study on the catalytic and biological activity of copper oxide nanoparticles synthesizing from Achillea millefolium, which presents great and significant results (in both catalytic and biological activities) based on a simple and green procedure.Conclusion: Comprehensive biomedical and catalytic investigation of the biosynthesized CuO-NPs showed the mentioned method leads to synthesis of more eco-friendly nanoparticles. The in vitro studies showed promising and considerable results, and due to the great stability of these nanoparticles in a green media, effective biological activity considered as an advantageous.Keywords: copper oxide nanoparticles, green synthesis, catalytic activity, antibacterial activity, antifungal activity