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Functional Analysis of Forkhead Transcription Factor Fd59a in the Spermatogenesis of Drosophila melanogaster

Authors :
Ting Tang
Mengyuan Pei
Yanhong Xiao
Yingshan Deng
Yuzhen Lu
Xiao-Qiang Yu
Liang Wen
Qihao Hu
Source :
Insects, Vol 15, Iss 7, p 480 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Spermatogenesis is critical for insect reproduction and is regulated by many different genes. In this study, we found that Forkhead transcription factor Fd59a functions as a key factor in the spermatogenesis of Drosophila melanogaster. Fd59a contains a conversed Forkhead domain, and it is clustered to the FoxD subfamily with other FoxD members from some insect and vertebrate species. Mutations in Fd59a caused swelling in the apical region of the testis. More importantly, fewer mature sperm were present in the seminal vesicle of Fd59a mutant flies compared to the control flies, and the fertility of Fd59a2/2 mutant males was significantly lower than that of the control flies. Immunofluorescence staining showed that the homeostasis of the testis stem cell niche in Fd59a2/2 mutant and Fd59a RNAi flies was disrupted and the apoptosis of sperm bundles was increased. Furthermore, results from RNA sequencing and qRT-PCR suggested that Fd59a can regulate the expression of genes related to reproductive process and cell death. Taken together, our results indicated that Fd59a plays a key role in the spermatogenesis of Drosophila.

Details

Language :
English
ISSN :
20754450
Volume :
15
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Insects
Publication Type :
Academic Journal
Accession number :
edsdoj.0725ada985c247ce807e0d07bf0de705
Document Type :
article
Full Text :
https://doi.org/10.3390/insects15070480