Back to Search
Start Over
Experimental Study on Compatibility of Human Bronchial Epithelial Cells in Collagen–Alginate Bioink for 3D Printing
- Source :
- Bioengineering, Vol 11, Iss 9, p 862 (2024)
- Publication Year :
- 2024
- Publisher :
- MDPI AG, 2024.
-
Abstract
- This paper reports an experimental study on the compatibility of human bronchial epithelial (HBE) cells in a collagen–alginate bioink. The compatibility was assessed using the culture well method with three bioink compositions prepared from a 10% alginate solution and neutralized TeloCol-10 mg/mL collagen stock solution. Cell viability, quantified by (live cell count—dead cell count)/live cell count within the HBE cell-laden hydrogel, was evaluated using the live/dead assay method from Day 0 to Day 6. Experimental results demonstrated that the collagen–alginate 4:1 bioink composition exhibited the highest cell viability on Day 6 (85%), outperforming the collagen–alginate 1:4 bioink composition and the alginate bioink composition, which showed cell viability of 75% and 45%, respectively. Additionally, the live cell count was highest for the collagen–alginate 4:1 bioink composition on Day 0, a trend that persisted through Days 1 to 6, underscoring its superior performance in maintaining cell viability and promoting cell proliferation. These findings show that the compatibility of HBE cells with the collagen–alginate 4:1 bioink composition was higher compared with the other two bioink compositions.
Details
- Language :
- English
- ISSN :
- 23065354
- Volume :
- 11
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0708b5a5c0754336ac5a2a1f747a823a
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/bioengineering11090862