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Experimental Study on Compatibility of Human Bronchial Epithelial Cells in Collagen–Alginate Bioink for 3D Printing

Authors :
Taieba Tuba Rahman
Nathan Wood
Yeasir Mohammad Akib
Hongmin Qin
Zhijian Pei
Source :
Bioengineering, Vol 11, Iss 9, p 862 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

This paper reports an experimental study on the compatibility of human bronchial epithelial (HBE) cells in a collagen–alginate bioink. The compatibility was assessed using the culture well method with three bioink compositions prepared from a 10% alginate solution and neutralized TeloCol-10 mg/mL collagen stock solution. Cell viability, quantified by (live cell count—dead cell count)/live cell count within the HBE cell-laden hydrogel, was evaluated using the live/dead assay method from Day 0 to Day 6. Experimental results demonstrated that the collagen–alginate 4:1 bioink composition exhibited the highest cell viability on Day 6 (85%), outperforming the collagen–alginate 1:4 bioink composition and the alginate bioink composition, which showed cell viability of 75% and 45%, respectively. Additionally, the live cell count was highest for the collagen–alginate 4:1 bioink composition on Day 0, a trend that persisted through Days 1 to 6, underscoring its superior performance in maintaining cell viability and promoting cell proliferation. These findings show that the compatibility of HBE cells with the collagen–alginate 4:1 bioink composition was higher compared with the other two bioink compositions.

Details

Language :
English
ISSN :
23065354
Volume :
11
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Bioengineering
Publication Type :
Academic Journal
Accession number :
edsdoj.0708b5a5c0754336ac5a2a1f747a823a
Document Type :
article
Full Text :
https://doi.org/10.3390/bioengineering11090862