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Genetic Signatures: CD44 Single-Nucleotide Polymorphisms Affect Cell Surface Expression and Elevate Risk in Head and Neck Squamous Cell Carcinoma

Authors :
Muhammad Kashif
Shah Jahan
Sadia Minhas
Ali Amar
Romeeza Tahir
Haseeb Nisar
Faheem Shehzad
Abdul Hanan Nagi
Nadeem Afzal
Source :
JCO Global Oncology, Iss 10 (2024)
Publication Year :
2024
Publisher :
American Society of Clinical Oncology, 2024.

Abstract

PURPOSEThis study aimed to investigate the impact of single-nucleotide polymorphisms (SNPs) in the CD44 gene, specifically in the 3'UTR region (rs13347) and intronic region (rs187115), on the cell surface expression of CD44 protein and the risk of development of head and neck squamous cell carcinoma (HNSCC).MATERIALS AND METHODSThe study involved analysis of 85 samples and 85 healthy controls. Immunohistochemistry (IHC) and flow cytometry were used to assess cell surface protein expression using CD44 antibody. DNA from formalin-fixed paraffin-embedded tissue sections was isolated and amplified using targeted primers. Sanger sequencing of the resultant amplified products was performed to determine the genotypes of the CD44 rs13347 and rs187115 SNPs. GTEx and RegulomeDB were queried to evaluate the genotypic effects of these variants on target gene expression and regulation.RESULTSA comparison between patients with HNSCC and healthy controls revealed a significant association between CD44 rs13347 and an increased risk of HNSCC in all the analyzed models, especially the TT genotype showed a significantly higher risk with an odds ratio of 8.69 (95% CI, 2.35 to 32.09; P = .0003). However, no significant association was found between CD44 rs187115 and HNSCC in any of the models analyzed (all P > .05). Other notable findings included significant associations between CD44 rs13347 genotype and age (P = .031), number of CD44-positive tumor cells (P = .049), CD44 staining intensity (SI; P = .039), and CD44 immunoreactivity score (IRS) status (P = .019).CONCLUSIONThe T allele and homozygous TT genotype of CD44 rs13347 SNP were associated with increased susceptibility to HNSCC and decreased proportion of CD44-positive tumor cells, low SI, and reduced IRS.

Details

Language :
English
ISSN :
26878941
Issue :
10
Database :
Directory of Open Access Journals
Journal :
JCO Global Oncology
Publication Type :
Academic Journal
Accession number :
edsdoj.06fb58fca4f742ba985ea44481ca097b
Document Type :
article
Full Text :
https://doi.org/10.1200/GO.24.00084