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A Biomimetic Chip with Dendrimer-Encapsulated Platinum Nanoparticles for Enhanced Electrochemiluminescence Detection of Cardiac Troponin I

Authors :
Yun Hui
Weijun Kong
Weiliang Shu
Zhiting Peng
Fengshan Shen
Mingyang Jiang
Zhen Xu
Tianzhun Wu
Wenhua Zhou
Xue-Feng Yu
Source :
Chemosensors, Vol 12, Iss 10, p 214 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

The measurement of cardiac troponin I (cTnI) is of vital importance for the early diagnosis of acute myocardial infarction. In this study, an enhanced electrochemiluminescent immunoassay for the highly sensitive and precise determination of cTnI was reported. A biomimetic chip with nepenthes peristome surface microstructures to achieve single-layer microbead arrays and integrated microelectrode arrays (MEAs) for ECL detection was microfabricated. Ru@SiO2 nanoparticles were prepared as signal amplificators labeling immunomagnetic beads. Dendrimer-encapsulated platinum nanoparticles (Pt DENs) were electrochemically modified on ITO MEAs. The resulting Pt DEN-modified ITO MEAs preserved good optical transparency and exhibited an approximately 20-fold ECL signal amplification compared to that obtained from bare ITO. The method made full use of the biomimetic chip with Pt DENs to develop single-layer immunomagnetic bead arrays with increasingly catalyzed electrochemical oxidation of the [Ru(bpy)3]2+–TPA system. Consequently, a limit of detection calculated as 0.38 pg/mL (S/N = 3) was obtained with excellent selectivity, demonstrating significant potential for the detection of cTnI in clinical diagnostics.

Details

Language :
English
ISSN :
22279040
Volume :
12
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Chemosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.06a8ce83e5884876b4cc30c239a6118a
Document Type :
article
Full Text :
https://doi.org/10.3390/chemosensors12100214