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Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope

Authors :
Tingting Jiang
Jordana M. Henderson
Kevin Coote
Yi Cheng
Hillary C. Valley
Xiao-Ou Zhang
Qin Wang
Luke H. Rhym
Yueying Cao
Gregory A. Newby
Hermann Bihler
Martin Mense
Zhiping Weng
Daniel G. Anderson
Anton P. McCaffrey
David R. Liu
Wen Xue
Source :
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Publication Year :
2020
Publisher :
Nature Portfolio, 2020.

Abstract

Cas9 base editors are promising tools for correcting pathogenic single nucleotide mutations. Here the authors chemically modify mRNA encoding the editor and the gRNA to enhance editing and broaden its application.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.063c642bedca45fb8d0878850da6e48f
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-020-15892-8