Chemical constituents of Pteris ensiformis from Guizhou

To study the chemical constituents of Pteris ensiformis, silica gel, gel, MCI, C18 and other column chromatography were used for separation and purification, and their structures were identified by 1H-NMR, 13C-NMR, MS, IR and othe spectral data; the anti-tumor and anti-coagulation activities of some monomers were screened by MTS, APTT, PT and TT. The results were as follows: (1) A total of 15 compounds were isolated from P. ensiformis, the compounds were 2-hydroxy-acetylpyrrole (1), N-(3-carboxypropyl)-2-acetylpyrrole (2), 3-hydroxy-2-methylpyridine (3), N-methylhydroxylamine (4), pterosin S 13-O-glucoside (5), obtupterosin C (6), ent-11α-hydroxy-15-oxokauran-19-oic acid (7), ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid (8), β-sitosterol (9), ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid-O-glucopyranoside (10), 5, 5′-dibutoxy-2, 2′-bifuran (11), 5, 5′-di (2-ethyl-hexyloxy)-2, 2′-bifuran (12), (-)-loliolide (13), succinic acid (14), fumaric acid (15). Compound 1 is a new natural product of pyrrole alkaloids. Compounds 1-7, 10-15 were isolated from P. ensiformis for the first time, and compounds 1, 3, 4 were isolated from Pteris for the first time. (2) The results of activity test showed that compounds 1, 2, 3, 5, 6 and 10 inhibited the growth of tumor cells HL-60, A549, SMMC-7721, MDA-MB-231 and SW480 in vitro at a concentration of 40 μmol·L-1, At the concentration of 2.0 mmol·L-1, compounds 1, 2, 3 and 6 shortened APTT and compounds 1, 5 and 6 prolonged PT. The study enriches the chemical constituents of P. ensiformis from Guizhou and provides a material basis for the development of anti-tumor drugs.