The Method of Sterile Anaerobic Centrifugation

The method of sterile anaerobic centrifugation was developed. The essence of the method is centrifuging the suspensions of bacteria or insoluble metal compounds in sealed flasks immersed in a water layer. Dipping flasks in water prevents destruction of the flasks under the centrifugal force. The method provides the possibility to centrifuge in anaerobic (inert gas) atmosphere. Thus, the method allows obtaining biomass of both aerobic and anaerobic microorganisms in a sterile conditions and preparing sterile insoluble metal compounds.