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Selegiline Differentiates Adult Stem Cells toward Dopaminergic-Like Neurons: A Comparison between Two Cellular Niches of Hippocampal Neurogenesis
- Source :
- Cell Journal, Vol 25, Iss 6, Pp 383-390 (2023)
- Publication Year :
- 2023
- Publisher :
- Royan Institute (ACECR), Tehran, 2023.
-
Abstract
- Objective: Neural stem cells (NSCs) are suitable therapeutic candidates. Here, we compare the proliferation rate,differentiation potential, and expression levels of specific markers in two groups of cultured NSCs derived from ratsubgranular (SGZ) and subventricular (SVZ) zones.Materials and Methods: In this experimental study, NSCs isolated from SGZ and SVZ were cultured in α-minimalessential medium (α-MEM) supplemented with 1% penicillin/streptomycin, 10% fetal bovine serum (FBS), 20 ng/mlbasic fibroblast growth factor (bFGF), 20 ng/ml epidermal growth factor (EGF), and B27 supplement. Glial fibrillaryacidic protein (Gfap), p75 neurotrophin receptor (Ngfr), tyrosine kinase receptor A (TrkA), beta-tubulin III (βTIII), andNestin gene levels were compared via reverse transcription polymerase chain reaction (RT-PCR) in these NSCs.Nestin and Gfap protein levels were compared by immunoassay. Subsequently, both populations were induced with10-8 M selegiline for 48 hours, followed by immunohistochemical analysis of tyrosine hydroxylase (TH) levels. One-wayANOVA and Tukey’s post-test were used with a significance level of P
Details
- Language :
- English
- ISSN :
- 22285806 and 22285814
- Volume :
- 25
- Issue :
- 6
- Database :
- Directory of Open Access Journals
- Journal :
- Cell Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.05c0a5d3c20f41fe810c2e144e18f705
- Document Type :
- article
- Full Text :
- https://doi.org/10.22074/cellj.2023.1987841.1214