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Zincophorin – biosynthesis in Streptomyces griseus and antibiotic properties

Authors :
Walther, Elisabeth
Boldt, Sabrina
Kage, Hirokazu
Lauterbach, Tom
Martin, Karin
Roth, Martin
Hertweck, Christian
Sauerbrei, Andreas
Schmidtke, Michaela
Nett, Markus
Source :
GMS Infectious Diseases, Vol 4, p Doc08 (2016)
Publication Year :
2016
Publisher :
German Medical Science GMS Publishing House, 2016.

Abstract

Zincophorin is a polyketide antibiotic that possesses potent activity against Gram-positive bacteria, including human pathogens. While a number of total syntheses of this highly functionalized natural product were reported since its initial discovery, the genetic basis for the biosynthesis of zincophorin has remained unclear. In this study, the co-linearity inherent to polyketide pathways was used to identify the zincophorin biosynthesis gene cluster in the genome of the natural producer HKI 0741. Interestingly, the same locus is fully conserved in the streptomycin-producing actinomycete IFO 13350, suggesting that the latter bacterium is also capable of zincophorin biosynthesis. Biological profiling of zincophorin revealed a dose-dependent inhibition of the Gram-positive bacterium . The antibacterial effect, however, is accompanied by cytotoxicity. Antibiotic and cytotoxic activities were completely abolished upon esterification of the carboxylic acid group in zincophorin.

Details

Language :
English
ISSN :
21958831
Volume :
4
Database :
Directory of Open Access Journals
Journal :
GMS Infectious Diseases
Publication Type :
Academic Journal
Accession number :
edsdoj.0497c0d1057243c6abce843b06e3fde7
Document Type :
article
Full Text :
https://doi.org/10.3205/id000026