Effects of arecoline and calcium ion on the permeability of dysplastic oral epithelia model in vitro

Objective To study the effects of arecolineand Calcium ion (Ca2+) on the permeability of dysplastic oral epithelia model in vitro. Methods To establish the dysplastic oral epithelia model in vitro by culturing oral keratinocyte (DOK) and harvesting a DOK cell monolayer. The models were divided into control group, arecoline group and "Ca2++ are⁃ coline" group. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treated⁃ with arecoline and Ca2+. Results In arecoline group, the lucifer yellow Papp values of each subgroup were higher than that of control group (P < 0.05), and the values increased as arecoline concentration elevated and time lasted (P < 0.05). When the "Ca2 ++ arecoline" group were pretreated with Ca2 +, the values of subgroup "10 μg/mL" was lower than that of the corresponding arecoline group (P > 0.05). However, the value of subgroup "4 h 10 μg/mL" of "Ca2++ arecoline" group had no statistical difference with that of control group (P > 0.05), while the other subgroups were increased (P < 0.05); Be⁃ sides, these values in "Ca2++ arecoline" group were increased as the arecoline concentration elevated and time lasted too (P < 0.05). Conclusion Intervention of arecoline contributes to the increase of permeability of DOK cell monolayer mod⁃ el, which maybe an important reason for the cancerization of dysplastic oral epithelia, however Ca2+might weaken these ef⁃ fects of arecoline in the process.