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Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-N-nitrosourea

Authors :
Keiko Inami
Yoshihisa Asada
Takumi Harada
Yuta Okayama
Noriko Usui
Masataka Mochizuki
Source :
Genes and Environment, Vol 41, Iss 1, Pp 1-9 (2019)
Publication Year :
2019
Publisher :
BMC, 2019.

Abstract

Abstract Background An extract from Spatholobus suberectus (S. suberectus) Dunn has been reported to show potent antimutagenic effects against N-alkyl-N-nitrosoureas in umu screening. The aim of this study was to identify the antimutagenic components from extracts of S. suberectus against N-methyl-N-nitrosourea (MNU) in the Ames assay with Salmonella typhimurium strain TA1535 and to elucidate the antimutagenic mechanism of the flavonoids. Results From the ethyl acetate fraction obtained from fractionation of the methanol extract of S. suberectus Dunn, medicarpin, formononetin and isoliquiritigenin were successfully isolated through a combination of normal- and reversed-phase chromatography. Genistein and naringenin, which were already reported to be contained in S. suberectus Dunn, were also tested for their antimutagenicity towards MNU, along with formononetin, isoliquiritigenin and medicarpin. Our results demonstrated that genistein, isoliquiritigenin, medicarpin and naringenin were antimutagenic against MNU without showing cytotoxicity. MNU is reported to cause not only DNA alkylation but also induce reactive oxygen species. The hydroxyl radical scavenging capacity of the flavonoids was correlated with the antimutagenic capacity, indicating that the hydroxyl radical scavenging activity was involved in their antimutagenicity towards MNU. Conclusions It is important to prevent DNA damage by N-nitrosamines for cancer chemoprevention. Genistein, isoliquiritigenin, medicarpin and naringenin were demonstrated to possess an antigenotoxic effects against carcinogenic MNU due to their radical scavenging activity.

Details

Language :
English
ISSN :
18807062
Volume :
41
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Genes and Environment
Publication Type :
Academic Journal
Accession number :
edsdoj.02963e1c6df049bf87a3990f1dfa4f28
Document Type :
article
Full Text :
https://doi.org/10.1186/s41021-019-0137-4