Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) infection in Candidate Bulls before taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)

Bovine Viral Diarrhea (BVD) is a common infection all over the world. It causes important economical losses in cattle breeding. In this study, a total of 46 blood samples were examined taken into tubes with and without EDTA from bulls in Artifical Insemination Centers. Blood serum samples were tested to detect for antibodies against Bovine Viral Diarrhea Virus (BVDV) and leukocyte samples were tested for BVDV antigens by ELISA methods. Eight (17.3%) out of 46 serum samples were found as positive by the means of antibodies against BVDV while 3 (6.5%) out of 46 leukocyte samples were detected as positive for BVD antigens. One (2.1%) of the seropositive bulls was detected as positive for BVD antigen. Two (4.3%) of the seronegative bulls were detected as positive for BVD antigens.