Back to Search
Start Over
Epigenome‐Wide Association Study for All‐Cause Mortality in a Cardiovascular Cohort Identifies Differential Methylation in Castor Zinc Finger 1 (CASZ1)
- Source :
- Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, Vol 8, Iss 21 (2019)
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background DNA methylation is implicated in many chronic diseases and may contribute to mortality. Therefore, we conducted an epigenome‐wide association study (EWAS) for all‐cause mortality with whole‐transcriptome data in a cardiovascular cohort (CATHGEN [Catheterization Genetics]). Methods and Results Cases were participants with mortality≥7 days postcatheterization whereas controls were alive with≥2 years of follow‐up. The Illumina Human Methylation 450K and EPIC arrays (Illumina, San Diego, CA) were used for the discovery and validation sets, respectively. A linear model approach with empirical Bayes estimators adjusted for confounders was used to assess difference in methylation (Δβ). In the discovery set (55 cases, 49 controls), 25 629 (6.5%) probes were differently methylated (P0.05); the SLC4A9 transcript did not pass quality control. The cg17944110 probe is located within a potential regulatory element; expression of predicted targets (using GeneHancer) of the regulatory element, UBIAD1 (P=0.01) and CLSTN1 (P=0.03), were lower in cases. Conclusions We identified 6 novel methylation sites associated with all‐cause mortality. Methylation in CASZ1 may serve as a regulatory element associated with mortality in cardiovascular patients. Larger studies are necessary to confirm these observations.
Details
- Language :
- English
- ISSN :
- 20479980
- Volume :
- 8
- Issue :
- 21
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0220683bd3ac4944a048c609bacd8f55
- Document Type :
- article
- Full Text :
- https://doi.org/10.1161/JAHA.119.013228